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KMID : 0364820210570030183
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Korean Journal of Microbiology
2021 Volume.57 No. 3 p.183 ~ p.196
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PHA synthesis by bacteria using low density polyethylene, starches and cellulosics
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Sohail Rafeya
Batool Rida
Jamil Nazia
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Abstract
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This study was conducted for optimization of polyhydroxyalkanoate (PHA) production using starches, cellulosics as carbon sources and mixed cultures. The capability of PHA producers to degrade and utilize LDPE (Low-density polyethylene) by carbon assimilation was also studied. Tannery effluents were used for isolation of PHA producers and screened using Nile blue and Nile red supplemented media. PHA production studies showed optimal PHA production occurred during 24 to 48 h time. Maximum PHA production was obtained at 24 h. After 48 h, gradual decline in PHA production was observed. Best candidate for PHA production was found to be strain PWF, based on number, size of granules inside cell, and %PHA production. PHA production was also optimized by use of mixed culture. Among starch-based sources, highest production rates were on pure starch. Among cellulose-based sources, maximum production rates were on dry wood powder instead of pure crystalline cellulose. In contrast, wood extract and wood shavings showed more pronounced PHA production rates, comparable to production on dry wood powder. LDPE utilization as sole carbon source in selective media showed that PHA producers were able to degrade synthetic plastic. Focus of future studies can be PHA production using these sources on industrial scale.
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KEYWORD
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Bacillus tequilensis, Pseudomonas aeruginosa, LDPE, polyhydroxyalkanoate, tannery
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